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KMID : 1025520080500050713
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Journal of Animal Science and Technology
2008 Volume.50 No. 5 p.713 ~ p.720
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Isolation and Identification of Hydrolytic Enzyme-producing Bacteria from Spent Mushroom Substrate
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Kim Young-Il
Jung Se-Hyung
Seok Joon-San
Yang Si-Yong
Huh Jeong-Weon
Kwak Wan-Sup
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Abstract
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This study was conducted to isolate and identify xylanase- and cellulase-producing thermophilic bacteria from stacked spent mushroom substrates and to determine the optimal medium conditions for their growth. Bacteria with the highest xylanase and CMCase activities were strain 3 and 201-7. Both of them were identified as Bacillus spp. and named Bacillus subtilis KU3 and Bacillus subtilis KU201-7. The optimal medium condition of Bacillus subtilis KU3 was obtained when 3%(w/v) of yeast extract and 1%(w/v) of maltose were used as nitrogen and carbon sources, respectively. That of Bacillus subtilis KU201-7 was obtained when 0.5%(w/v) of yeast extract and 0.5%(w/v) of CMC were used as nitrogen and carbon sources, respectively.
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KEYWORD
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Xylanase, CMCase, Spent mushroom substrates, Enzyme activity
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